Journal: Nature Communications

Article Title: PD-L1 + and XCR1 + dendritic cells are region-specific regulators of gut homeostasis

doi: 10.1038/s41467-021-25115-3

Figure Lengend Snippet: a Flow cytometry dot plots from duodenal and colonic human biopsies showing DC phenotype, and immunofluorescence showing CD1c and CD141 colocalization in colonic biopsies (20X). b Graphs represent the percentage of PD-L1 + and XCR1 + DCs gated on singlets, live, HLA-DR High , dump − (CD19, CD3, CD14) and CD11c + cells; N = 8, paired two-tailed Student’s t test, * p = 0.031. Source data are provided as a Source Data file. Biorender was used for illustration.

Article Snippet: For CD141 and CD1c co-staining, anti-CD141 (E7Y9P; 1:1000; Cell Signaling Technology) and anti-CD1c (EPR23189-196; 1:250; Abcam) were used as primary antibodies, and AF594 (ab150080) and AF647 (ab150075) (both from Abcam) were used as secondary antibodies.

Techniques: Flow Cytometry, Immunofluorescence, Two Tailed Test

Journal: Nature Communications

Article Title: PD-L1 + and XCR1 + dendritic cells are region-specific regulators of gut homeostasis

doi: 10.1038/s41467-021-25115-3

Figure Lengend Snippet: a Flow cytometry dot plots from duodenal and colonic human biopsies showing DC phenotype, and immunofluorescence showing CD1c and CD141 colocalization in colonic biopsies (20X). b Graphs represent the percentage of PD-L1 + and XCR1 + DCs gated on singlets, live, HLA-DR High , dump − (CD19, CD3, CD14) and CD11c + cells; N = 8, paired two-tailed Student’s t test, * p = 0.031. Source data are provided as a Source Data file. Biorender was used for illustration.

Article Snippet: For CD141 and CD1c co-staining, anti-CD141 (E7Y9P; 1:1000; Cell Signaling Technology) and anti-CD1c (EPR23189-196; 1:250; Abcam) were used as primary antibodies, and AF594 (ab150080) and AF647 (ab150075) (both from Abcam) were used as secondary antibodies.

Techniques: Flow Cytometry, Immunofluorescence, Two Tailed Test